Electrokinetic Trapping

Sandia National Laboratory, Livermore

We have developed a novel technique for concentrating proteins from dilute solutions using electrokinetic trapping, in which charged macromolecules are reversibly trapped in a microchannel packed with porous silica particles under an applied electric field.

This technique provides a simple, on-column method of preconcentration of proteins and other charged macromolecules that can be easily integrated with a miniaturized separation device and requires changes in neither solvent, buffer, nor flow direction. A model protein, ovalbumin, has been concentrated by over two orders of magnitude using this technique.

Upcoming Presentations

A.K. Singh, D.J. Throckmorton, B.J. Kirby
" A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Trapping," HPCE 2003, San Diego, CA.

References

A.K. Singh, D.J. Throckmorton, B.J. Kirby, A.P. Thompson
" A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Trapping and Release," in MicroTAS 2002, p. 347-349, 2002.