Green-labeled macrophages were sorted from a 40/60 ratio mixture at a throughput of 22 cells/s over 30 min achieving a 93% sorting purity and a 60% recovery yield.
| μFACS performances at different ratios of green- to red-labeled macrophages. | ||||
Green cells / red cells |
Throughput [cells/s] |
Recovery [%] |
Purity [%] |
Cell number |
|---|---|---|---|---|
90/10 |
16 |
55 ± 5 |
97 ± 3 |
21,698 |
40/60 |
22 |
60 ± 10 |
93 ± 3 |
38,877 |
10/90 |
14 |
63 ± 9 |
75 ± 10 |
24,847 |
The initial cell density was set at 4x106 cells/mL before loading the cells into the chip. Recovery and purity were determined by fluorescence microscopy (errors set as the standard deviation of 5 fields of view); throughput and cell number were measured in real time. |
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Perroud, T. D.; Kaiser, J. N.; Sy, J. C.; Lane, T. W.; Branda, C. S.; Singh, A. K.; Patel, K. D. Microfluidic-Based Cell Sorting of Francisella tularensis Infected Macrophages using Optical Forces. Analytical Chemistry 80, 6365-6372 (2008).