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MicroChemLab™ biodetector

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• Glenn Kubiak,
  925.294.3375

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Victoria VanderNoot, PhD.

Analytical Chemist

Senior Member of Technical Staff,
Biosystems Research Department

Biographical

I was educated in Canada and received my Ph.D. in Analytical Chemistry from Carleton University in Ottawa.

I did a joint academic/industrial post-doc, splitting my time between the University of Colorado Health Sciences Center in Denver and Monsanto Company in St. Louis.

From there I went to the University of Iowa (participating in joint research between the Departments of Chemical and Biochemical Engineering and Medicinal and Natural Products Chemistry) before joining Sandia in 1998.

Current Research Interests

I am currently engaged in research on several projects in the area of protein separation and detection. The first project is the development of the µChemLab™ chip-based platform for the separation and detection of protein toxins funded under the DOE’s Chemical and Biological Non-Proliferation program. As part of a large interdisciplinary team, we have designed, built and tested a two-channel prototype that houses all of the high voltage control, the liquid handling, laser-induced fluorescence detection and analysis components in a small, stand-alone box. I have worked primarily to adapt capillary gel electrophoresis to a microfluidic format and to help optimize fluorescent labeling conditions. Ongoing research focuses on developing new chip-based separations as well as the adaptation of this technology and other microseparations to more complex biological systems, specifically in the area of biodefense.

I am also engaged in an NIH (NIDCR - National Institute of Dental and Craniofacial Research) funded project to develop technologies for the detection of biomarkers in saliva. In collaboration with researchers at the University of Michigan College of Engineering and School of Dentistry, we are developing techniques to look for biological signatures that correlate with periodontal disease. Some of these same markers may also be indicators of other systemic diseases and could ultimately aid in early detection of pathogen exposure.

An ongoing area of interest focuses on immune response and cellular signaling. Our research focuses on both cytokines and the phosphorylated proteins associated with cellular signaling events. Our approach makes use of our strong expertise in protein separations and detection and comprises the selective affinity purification of phosphorylated proteins from immune cells and the fingerprinting of the phosphorylated protein component in response to a variety of stimuli. Past collaborations include the University of New Mexico Medical Center and the Alliance for Cellular Signaling located at the University of Texas, Southwestern.

Additional research interests include the interaction of toxins with their receptors probed by Surface Plasmon Resonance, the development of novel approaches to isoelectric focusing of proteins and the development of affinity chromatographic phases for protein biotoxins

Selected Publications and Presentations

• Victoria VanderNoot, Shelly Pizarro, Julie Fruetel, Ron Renzi, and Jamie Stamps,
  Phosphoprotein Signatures for Early Pathogen Exposure Detection Using µChemLab™,
  Mainstreaming Microfluidics, Boston, MA, May 15-16, 2003.
  
• M. VanRollins and V.A. VanderNoot,
  Simultaneous Resolution of Underivatized Regioisomers and Stereoisomers of Arachidonate Epoxides by Capillary Electrophoresis Analytical Biochemistry, (2003), 313, 106-116.
  
• Victoria VanderNoot, Boyd Wiedenman, Yolanda Fintschenko and Julie Fruetel,
  Incorporation of Sample Preconcentration into the µChemLab™/CB Platform for Enhanced Sensitivity,
  16th International Symposium on Microscale Separations and Analysis (HPCE 2003), San Diego, CA, January 17-22, 2003.
  
• Victoria A. VanderNoot and Mike VanRollins,
  Capillary Electrophoresis of Cytochrome P-450 Epoxygenase Metabolites of Arachidonic Acid. 2. Resolution of Stereoisomers Analytical Chemistry, (2002), 74, 5866-5870.
  
• Victoria A. VanderNoot and Mike VanRollins,
  Capillary Electrophoresis of Cytochrome P-450 Epoxygenase Metabolites of Arachidonic Acid. 1. Resolution of Regioisomers Analytical Chemistry, (2002), 74, 5859-5865.
  
• Julia A. Fruetel, Brent A. Horn, Jay A.A. West, James F. Stamps, Victoria A. VanderNoot, Mary Clare Stoddard, Ronald F. Renzi and Debbie Padgen,
  Results From µChemLab™/CB, A Portable System For Detecting Chemical And Biological Warfare Agents.
  µTAS 2002. Y. Babu, S. Shoji and A. van den Berg. ed.s,
  (Kluwer Academic, Dordrecht), (2002), pp. 524-526.
  
• Ronald F. Renzi, James F. Stamp, Robert W. Crocker, Boyd J. Wiedenman, Scott M. Ferko, Brent A. Horn, Dan Yee, Victoria A. VanderNoot, Jason A. West, Julia A. Fruetel, Yolanda Fintschenko and Mary Clare Stoddard,
  Engineered Improvement of the Generation-2 µChemLab‘ Biotoxin Detector. µTAS 2002.
  Y. Babu, S. Shoji and A. van den Berg. ed.s,
  (Kluwer Academic, Dordrecht), (2002), pp. 703-705.
  
• Shelly A. Pizarro, Joseph S. Schoeniger, Victoria A. VanderNoot.
  Capillary-Based Separation Microtechnologies For Pattern Profiling Of Phosphoproteins Involved In T-Cell Signaling.
  Microtechnologies in Medicine and Biology 2002 (IEEE-EMBS) Proceedings, A. Dittmar and D. Beebe, ed.s,
  (IEEE, Inc, Piscataway, NJ), (2002), pp.433-436.
  
• Julia Fruetel, V. A. VanderNoot, Brian Kirby, Charlie Hasselbrink and Tim Shepodd
  Polymeric Coating Protocol for µChemLab/CB.
  15^th International Symposium on Microscale Separations and Analysis (HPCE 2002), Stockholm, Sweden, April 13-18, 2002.
  
• Victoria A. VanderNoot, Emily Keuhler And Shelly Pizarro,
  Isoelectric Focusing With Laser-Induced Fluorescence Detection Of Phosphoprotein Signatures,
  15^th International Symposium on Microscale Separations and Analysis,
  (HPCE 2002), Stockholm, Sweden, April 13-18th, 2002.
  
• VanderNoot VA, Hux G, Schoeniger J, Shepodd T.
  "Isoelectric Focusing Using Electrokinetically-Generated Pressure Mobilization".
  µTAS (2001). van den Berg, et al. ed.s,
  (Kluwer Academic, Amsterdam), pp. 127-128.
  
• Fruetel J, Renzi R, Crocker R, VanderNoot V, Stamps J, Yee D, Rakestraw D, Paul P, Arnold D, Bailey, C.
  " µChemLab/CB: Parallel Channel Analysis in an Integrated Microchip Device."
  14^th International Symposium on Microscale Separations and Analysis
  (HPCE 2001), Boston, MA, January 13-18, 2001.
  
• Fruetel, J.R., R.; Crocker, R.; VanderNoot, V.; Ferko, S.; Stamps, J.; Shokair, I.; Yee, D.; Hasselbrink, C.,
  "Development Of µChemlab/CB For Detection Of Biotoxins". Proceedings of the First Joint Detection for Chemical and Biological Defense, (2000): p. 131-139.
  
• VanderNoot VA, Volponi J, Hebbring S, Schoeniger JS.
  "Development and Characterization of Receptor Based Stationary Phases for Capillary Affinity Electrochromatography."
  Federation of Analytical Chemistry and Spectroscopy Societies Conference, Vancouver, B.C., Canada, October 25-29. 1999
  
• Caldwell EE, Andreasen AM, Blietz MA, Serrahn JN, VanderNoot V, Park Y, Yu G, Linhardt RJ, Weiler JM.
  "Heparin binding and augmentation of C1 inhibitor activity."
  Arch Biochem Biophys 361(2):215-22. 1999.
  
• Fath M, VanderNoot V, Kilpelainen I, Kinnunen T, Rauvala H, Linhardt RJ.
  "Interaction of soluble and surface-bound heparin binding growth-associated molecule with heparin."
  FEBS Lett 454(1-2):105-8. 1999.
  
• I. Capila, V.A. VanderNoot, T.R. Mealy, B.A. Seaton, R.J. Linhardt,
  Interaction of Heparin with Annexin V. FEBS Letters, (1999), 446, 327-330.
  
• Lai EPC, Farfara A, VanderNoot VA, Kono M, Polsky B.
  "Surface Plasmon Resonance Sensors Using Molecularly Imprinted Polymers for Sorbent Assay of Theophylline, Caffeine and Xanthine."
  Canadian J Chem 76:265-273. 1998.
  
• J. H. Choe, V. A. VanderNoot, R. J. Linhardt and J. S. Dordick,
  Affinity-based Reversed Micellar Extraction and Separation (ARMES) to Resolve Structurally Similar Glycoproteins.
  AICHE Journal, (1998), 44, 2542-2548.
  
• VanderNoot VA, Hileman RE, Dordick JS, Linhardt RJ.
  "Affinity capillary electrophoresis employing immobilized glycosaminoglycan to resolve heparin-binding peptides."
  Electrophoresis 19(3):437-41. 1998.
  
• VanderNoot VA, Dordick JS, Van Rollins M.
  " Capillary electrophoresis for the enantiomeric and regioisomeric resolution of fatty acid epoxides in biological samples."
  Federation of Analytical Chemistry and Spectroscopy Societies Conference, Providence, RI. October 26-30. 1997.
  
• J. H. Choe, V. A. VanderNoot, R. J. Linhardt and J. S. Dordick,
  Parameters Affecting the Affinity Based Reversed Micellar Extraction and Separation of Glycoproteins.
  Biotechnology Progress, (1997), 13, 440-445.
  
• C.L. Chakrabarti, B. Marchand, V. A. VanderNoot, J. Walker and W.H. Schroeder,
  Development Of A New Method For Direct Determination Of Selenium Associated With Atmospheric Particulate Matter Using Chemical Modifiers And Graphite Probe Furnace Atomic-Absorption.
  Spectrochimica Acta Part B-Atomic Spectroscopy, (1996), 51, 155-163.
  
• VanderNoot VA, Fitzpatrick FA.
  "Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding protein 2." Anal Biochem 230(1):108-14. 1995.
  
• Berg DA, VanderNoot VA, Barradas RG, Lai EPC.
  "In-Situ Photoacoustic Determination of Thallium Underpotential Deposition on Copper." J Electroanal Chem 369:33-37. 1994.
  
• VanderNoot VA, Lai EPC.
  "Determination of Mercury (II) in Dithizone-impregnated Latex Microparticles by Photochromism-induced Photoacoustic Spectroscopy"
  Anal Chem 64:3187-3190. 1992.
  
• VanderNoot VA, Lai EPC.
  "Detecting Surface Plasmon Resonance Using Photothermal Deflection Spectroscopy: Development of an Optical Immunosensor."
  Spectroscopy 6(7):28-33. 1991.

 

For More Information Contact: Victoria VanderNoot